Shotgun Proteomics: Methods and Protocols (Methods in Molecular Biology, Book 1156)
Shotgun Proteomics: Methods and Protocols (Methods in Molecular Biology, Book 1156) by Daniel Martins-de-Souza
English | 2014 | ISBN: 1493906844 | ISBN-13: 9781493906840 | 492 pages | PDF | 8,7 MB
English | 2014 | ISBN: 1493906844 | ISBN-13: 9781493906840 | 492 pages | PDF | 8,7 MB
Shotgun Proteomics: Methods and Protocols serves as a vital collection of protocols through which thousands of proteins can be simultaneously identified, quantified and characterized in a high throughput manner.
Beginning with the history of proteomics centered on the vital role of mass spectrometry in its development, this detailed volume continues with chapters on sample pre-fractionation, in vivo and in vitro stable isotope labeling, label-free proteomics, informatics, protein-protein interactions, targeted proteomics and post-translational modifications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Practical and comprehensive, Shotgun Proteomics: Methods and Protocols is an ideal and up-to-date guide for researchers seeking to understand the proteome of any given species.