Fast Detection of DNA Damage: Methods and Protocols (Methods in Molecular Biology)
Fast Detection of DNA Damage: Methods and Protocols (Methods in Molecular Biology) by Vladimir V. Didenko
English | 2 Aug. 2017 | ISBN: 1493971859 | 228 Pages | PDF | 6.64 MB
English | 2 Aug. 2017 | ISBN: 1493971859 | 228 Pages | PDF | 6.64 MB
This volume presents a comprehensive collection of quick assays for the detection of nuclear and mitochondrial DNA damage and its effects in live and fixed cells and tissues, and in bacterial genomes. Although, such rapid techniques are in demand in the “research trenches” they are not covered well in the literature. This volume is the first such compendium of the time-saving techniques for detection of DNA damage and its direct physiological outcomes including apoptosis, necrosis and phagocytic clearance. The volume demonstrates all levels of detection, starting from the molecular level up to the level of the entire live organism. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.