Argonaute Proteins: Methods and Protocols (Methods in Molecular Biology)
Argonaute Proteins: Methods and Protocols (Methods in Molecular Biology) by Katsutomo Okamura
English | 12 Nov. 2017 | ISBN: 149397338X | 260 Pages | EPUB | 3.18 MB
English | 12 Nov. 2017 | ISBN: 149397338X | 260 Pages | EPUB | 3.18 MB
This volume covers methods that analyze various Argonaute proteins from a variety of organisms to help researchers better understand their properties ranging from a molecular level to an organismal level. The chapters in this book explore the following topics: identification and expression analysis of guide nucleic acids and their targets; analysis of biochemical properties of Argonautes; biological functions of Argonautes; and obtaining materials and setting up analysis platforms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.