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Protein Complex Assembly: Methods and Protocols

Posted By: hill0
Protein Complex Assembly: Methods and Protocols

Protein Complex Assembly: Methods and Protocols (Methods in Molecular Biology) by Joseph A. Marsh
English | 2 May 2018 | ISBN: 149397758X | 524 Pages | PDF (True) | 23.23 MB

This volume details the importance of multiple experimental techniques and computational methods needed to obtain the comprehensive picture of protein complex structure, dynamics and assembly afforded by the emerging field of integrative structural biology. Chapters guide readers through the broad spectrum of approaches required for a complete representation of protein complexes, including expression and purification, experimental characterization of structure and assembly, and computational methods for identifying protein complexes and modelling their assembly. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.