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Second Harmonic Generation Imaging (Series in Cellular and Clinical Imaging) (Repost)

Posted By: insetes
Second Harmonic Generation Imaging (Series in Cellular and Clinical Imaging) (Repost)

Second Harmonic Generation Imaging (Series in Cellular and Clinical Imaging) By Francesco S. Pavone, Paul J. Campagnola
2013 | 465 Pages | ISBN: 1439849145 | PDF | 16 MB


Second-harmonic generation (SHG) microscopy has shown great promise for imaging live cells and tissues, with applications in basic science, medical research, and tissue engineering. Second Harmonic Generation Imaging offers a complete guide to this optical modality, from basic principles, instrumentation, methods, and image analysis to biomedical applications. The book features contributions by experts in second-harmonic imaging, including many pioneering researchers in the field. Written for researchers at all levels, it takes an in-depth look at the current state of the art and possibilities of SHG microscopy. Historically, clinical imaging at the cellular and tissue level has been performed by pathologists on ex vivo biopsies removed by the surgeon. While histology remains the "gold standard" for pathologists, its interpretation remains highly subjective. Much of SHG research has focused on developing more quantitative, objective metrics. A tutorial for newcomers and an up-to-date review for experts, this book explores how SHG may be used to more precisely image a wide range of pathological conditions and diseases.