Become A Master Hplc - Analysis Specialist Training

Are you ready to become an HPLC expert? 21 different analysis applications, 24 comprehensive courses, in the laboratory

What you'll learn

Comprehensive HPLC Knowledge: Participants will gain in-depth knowledge of HPLC technology.

Expert Training: Our expert trainers will guide participants in equipment usage, solution and analyte preparation, and analysis with different detectors.

Methodology and Process Understanding: A solid understanding of the methodologies and processes used in HPLC analysis.

Software Proficiency: Participants will develop proficiency in using the software associated with HPLC devices.

Problem-Solving Skills: Training will equip participants with the tools to address various challenges that may arise during analysis.

Independent Analysis: By the program's conclusion, participants will have the knowledge and confidence to conduct HPLC analysis independently.

Nuanced Understanding: Participants will grasp the intricacies of HPLC analysis, enabling them to analyze with confidence.

Requirements

Basic Knowledge of Chemistry, About Analysis, and Math

Description

BEST ONLINE HPLC TRAINING IN THE WORLD!High Performance Liquid Chromatography (HPLC) is a widely used analytical technique in the field of chemistry and biology. It is used to separate, identify and quantify components in a mixture. With the increasing demand for high-quality analysis, it is important to have the necessary skills and knowledge to operate HPLC equipment and interpret results accurately. This is where our HPLC Training comes in.Our comprehensive training program is designed to provide you with a complete understanding of HPLC and its applications. Whether you are a beginner or an experienced analyst, our courses offer a comprehensive understanding of the principles and practices of HPLC. You will learn the logic of HPLC, such as how the device works, what the different parts of the equipment are and how to operate it safely.One of the key features of our HPLC Training is that it is taught in a laboratory setting. This means you will see hands-on work with the equipment and learn how to apply a variety of analytical techniques. Also, our program includes 21 different analysis applications, so you can familiarize yourself with the various applications of HPLC and learn how to apply them in different scenarios.Throughout the training, you will be taught by expert trainers who have years of experience in HPLC analysis. They will guide you through every step of the process, from the preparation of solutions and analytes to the analysis itself. You will learn how to analyze with different detectors, understand the different types of problems that may arise and how to solve them.Our HPLC Training program also covers the software part of the device, so you will learn how to navigate and use the software to analyze data effectively. This includes mastering the calibration process, calibration curve creation, and the reporting and printing process.By the end of the training, you will have a thorough understanding of HPLC and be able to analyze independently with HPLC devices. You will also have the necessary skills to manage a laboratory and be a lab manager. Whether you are looking to advance your career in the field of chemistry or biology, or simply want to gain a deeper understanding of HPLC, our training program is the perfect solution. Enroll now and take the first step towards becoming an HPLC expert.

Overview

Section 1: Mobile Phase And Washing Solutions

Lecture 1 What is the Mobile Phase?

Lecture 2 Wash Solvents and Lines

Lecture 3 Recommendations For Different Washing Systems

Lecture 4 How to Connect Mobile Phase to the System

Lecture 5 Reverse Phase and Normal Phase

Section 2: HPLC Pump Module

Lecture 6 Operation System of Pump Module

Lecture 7 How to Operate the Pump System?

Lecture 8 How Should We Remove Air Bubbles Formed in the System?

Lecture 9 The Liquid Sensor

Lecture 10 The Pump Module in Different Brands of Devices

Section 3: Auto Sampler Module

Lecture 11 Auto Sampler Module

Lecture 12 Sample Vials

Lecture 13 How Does Work the Injection System

Section 4: Column Oven Module

Lecture 14 Column Oven

Lecture 15 The Column Advice For Wash Process

Lecture 16 HPLC Columns

Lecture 17 How Assembled The Column?

Lecture 18 What is the Guard Column?

Section 5: Preliminary Information About HPLC Detectors and Software System

Lecture 19 Detectors Used In HPLC Devices

Lecture 20 Let’s Review The Software Program

Section 6: Things to Do Before Analysis

Lecture 21 Recommendations – Before Starting the Analysis

Lecture 22 How Much Should the Mobile Phase Amount Be?

Lecture 23 Preparation of the Mobile Phase

Lecture 24 Recommendations

Lecture 25 Things to Do Before Connecting the Mobile Phase and Washing Solutions

Lecture 26 Characteristics of Columns

Lecture 27 Pressure Problems Related to Columns

Lecture 28 Operation and Washing Processes of The Device – Auto Purge 1

Lecture 29 Cleaning the Pump Module

Lecture 30 Starting Flow in the Pump Module

Lecture 31 Connecting the Mobile Phase and Washing Solutions to the Device

Section 7: Preparation of Samples and Analytes

Lecture 32 Working Logic with Solutions

Lecture 33 Preparation of Reference Solutions

Lecture 34 Preparation of Stock Solution

Lecture 35 Preparation of Sample Solution

Lecture 36 Preparation of Stock Standard Solutions

Lecture 37 Preparation of Vials

Lecture 38 Placing Vials on the Tray

Section 8: Method Creation and Method Definition In The Software

Lecture 39 New method

Lecture 40 Creating the Batch Table

Section 9: How to Analyze with HPLC Device?

Lecture 41 Conditioning the Device and Column

Lecture 42 Starting to Analysis

Lecture 43 Setting the Shutdown Washing Mode

Section 10: Determination of Retention Times

Lecture 44 Determination of Retention Times of Reference Standards (Automatic Integration)

Lecture 45 Determination of Retention Times of Reference Standards (Manual Integration)

Lecture 46 Determination of Vitamin C Standard

Lecture 47 Determination of Vitamins B1, B3, B6

Section 11: Calculation of Actual Concentrations

Lecture 48 Calculation of Concentrations

Section 12: Processing the Analysis

Lecture 49 Introduction to Processing Analysis Results

Lecture 50 Getting Started with the Process

Lecture 51 Processing the Batch Table

Lecture 52 Calling the Batch Table

Lecture 53 Adding Parameters to the Result

Section 13: Displaying the Calibration Curves

Lecture 54 Displaying the Curves

Section 14: Calculation Process

Lecture 55 Calculation

Section 15: Reporting, Calibration Curve And Print Process

Lecture 56 Control of Concentrations Before Reporting

Lecture 57 Report Preparation

Lecture 58 Calibration Curve Deviate

Lecture 59 Printing in a Different Format

Lecture 60 Setting Peaks in a Report

Section 16: Analysis in a Gradient System

Lecture 61 Analysis in a Gradient System

Section 17: Washing Processes / Auto Purge

Lecture 62 Operation and Washing Processes of The Device Auto Purge Lesson 1

Lecture 63 Washing Processes Auto Purge – Lesson 2

Section 18: Mixing of Mobile Phases in Different Ways

Lecture 64 Mixing in the Pump Line

Lecture 65 Comparing Mixtures

Section 19: Modifying the Method And Batch While the Analysis Is In Progress

Lecture 66 Modifying the Method While the Analysis Is In Progress

Lecture 67 Making Changes to the Batch Table While Analysis Is In Progress

Section 20: Diode Array Detector || Analysis And Interpreting Results

Lecture 68 Preparing Device and Lines Before Analysis in Diode Array Detector

Lecture 69 Analysis in a Diode Array Detector

Lecture 70 Interpreting Results in a Diode Array Detector

Section 21: Analysis With RID Detector

Lecture 71 Analysis in RID Detector

Section 22: Different Columns With The Same Feature

Lecture 72 Analysis in Fluorescent Detector

Section 23: Different Columns With The Same Feature

Lecture 73 Analysis with 25 cm Long Column

Lecture 74 Long Column vs. Short Column

Section 24: Problems That May Be Encountered And Solution Methods

Lecture 75 Tailing Problem

Lecture 76 Resolution – Dividing Peak Retention Times

Lecture 77 If Parameters Change

Lecture 78 If Flow Rate Changes

Lecture 79 If the Wavelength Changes

Lecture 80 If the UV Detector Lamp Stays Off

Lecture 81 If Column Oven Temperature Changes

Lecture 82 Lamp Life of UV Detector

Lecture 83 If There Is a Difference in Mobile Phase Ratio

Laboratory Professionals,Students,Method Develeopers,Scientists,Analysts and laboratory personnel aiming to acquire troubleshooting skills to address potential issues.,Professionals working in laboratory environments who want to enhance their management skills.,Participants interested in learning the pre-analytical steps and precautions required in HPLC.,Individuals who wish to have a thorough understanding of the methodologies and processes involved in HPLC analysis.,Anyone who wants to become master at using HPLC software for data analysis.,Analysts and scientists aiming to gain proficiency in analyzing samples with different types of detectors.,Those seeking knowledge about the preparation of solutions and analytes for HPLC analysis.,Individuals who want to learn about HPLC equipment and its proper usage.,Professionals looking to gain a comprehensive understanding of HPLC analysis techniques.