Mastering Lc-Ms/Ms: Instrumentation And Diverse Applications

From Fundamentals to Frontiers: A Step-by-Step Journey Through LC-MS/MS Technology and Its Real-World Uses

What you'll learn

Build a solid foundation in LC-MS/MS theory and instrumentation.

Explain and compare major ionization techniques and mass analyzers.

Develop practical skills in sample preparation, instrument setup, and troubleshooting.

Train students in method development, calibration, and validation for LC-MS/MS.

Enable interpretation and processing of qualitative and quantitative LC-MS/MS data.

Highlight advanced applications and emerging trends in LC-MS/MS technology.

Requirements

Analytical chemistry

Description

This course, Mastering LC-MS/MS: Instrumentation and Diverse Applications, offers a comprehensive foundation in the principles, instrumentation, and methodologies of Liquid Chromatography coupled with Tandem Mass Spectrometry (LC-MS/MS). Participants will explore essential concepts in liquid chromatography and mass spectrometry, including their integration into a unified analytical workflow for accurate and efficient compound identification. Key topics include method development, system operation, calibration, maintenance, and practical troubleshooting strategies. Emphasis is placed on the mechanisms and characteristics of major ionization techniques such as Electrospray Ionization (ESI), Atmospheric Pressure Chemical Ionization (APCI), and Matrix-Assisted Laser Desorption/Ionization (MALDI), offering practical comparisons to help learners select the most appropriate technique based on analytical needs. Learners will also examine mass analyzers—Quadrupole, Time-of-Flight (TOF), Ion Trap, and Orbitrap—comparing their resolution, accuracy, speed, and suitability for diverse applications. The course introduces data acquisition modes and software tools, while showcasing real-world applications in pharmaceutical development, environmental analysis, food safety testing, and forensic investigations. Designed for scientists, laboratory analysts, and students, this course builds both theoretical knowledge and hands-on competence in LC-MS/MS for qualitative and quantitative workflows. LC-MS/MS ensures precision, sensitivity, reproducibility, quantification, validation, optimization, selectivity, integration, and workflow robustness daily. With structured modules, engaging visuals, and accessible explanations, the course prepares learners to apply LC-MS/MS confidently across multiple disciplines and professional contexts

Overview

Section 1: Introduction

Lecture 1 Introduction

Lecture 2 Introductory Video

Section 2: Hard vs Soft Ionization Mass Spectrometry

Lecture 3 Hard vs Soft Ionization Mass Spectrometry

Section 3: Instrument Fundamentals & Types of MS

Lecture 4 Instrument Fundamentals & Types of MS

Section 4: Mass Analyzer

Lecture 5 Mass Analyzers

Section 5: Fundamentals of LC-MS Operation

Lecture 6 Fundamentals of LC-MS Operation

Section 6: Troubleshooting and Maintenance

Lecture 7 Troubleshooting and Maintenance

Section 7: Troubleshooting and Maintenance

Lecture 8 Troubleshooting and Maintenance

Section 8: Hands-on the LC-MS Instrument

Lecture 9 Application-Examples

Section 9: Practical LC-MS Applications

Lecture 10 Application-Example 2

Section 10: Application-Example

Lecture 11 Application-Example 3

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