Ancestral Sequence Reconstruction (Repost)
David A. Liberles, "Ancestral Sequence Reconstruction"
English | 2007-07-26 | ISBN: 0199299188 | 267 pages | PDF | 5.7 mb
English | 2007-07-26 | ISBN: 0199299188 | 267 pages | PDF | 5.7 mb
comparative genomics. As a powerful technique for both testing evolutionary and ecological hypotheses as well as uncovering the link between sequence and molecular phenotype, there are potential applications in a number of fields. Beginning with a historical overview of the field including apllications, the discussion then moves into potential applications in drug discovery and the pharmaceutical industry. A section on computational methodology provides a detailed discussion on available methods for reconstructing ancestral sequences, including advantages,disadvantages, and potential pitfalls. Purely computational applications, including whole proteome reconstruction are discussed. Another section provides a detailed discussion on taking computationally reconstructed sequences and synthesizing them in the laboratory, while the last section describes scientific questions where experimental ancestral sequence reconstruction coupled to a computaional and experimental how-to guide, while simultaneously addressing some of the hot topics in the field.