Mouse Embryogenesis: Methods and Protocols
Mouse Embryogenesis: Methods and Protocols (Methods in Molecular Biology) by Paul Delgado-Olguin
English | 4 Apr. 2018 | ISBN: 149397713X | 208 Pages | PDF | 7.89 MB
English | 4 Apr. 2018 | ISBN: 149397713X | 208 Pages | PDF | 7.89 MB
This volume explores protocols for identifying mutant mice and characterizing parts of their anatomical, functional, cellular, and molecular phenotypes. The chapters in this book look at anatomical and functional phenotyping using quantitative imaging, isolation of specific embryonic cell types for cell culture, analysis of gene expression, and how to define chromatin structure. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.