Cellular Senescence: Methods and Protocols
Cellular Senescence: Methods and Protocols
by Marco Demaria
English | 2019 | ISBN: 1493989308 | 253 Pages | PDF | 6.57 MB
by Marco Demaria
English | 2019 | ISBN: 1493989308 | 253 Pages | PDF | 6.57 MB
This book describes current methods for the identification and characterization of the major hallmarks of senescent cells. Chapters focus on the high heterogeneity of the senescence phenotypes, and techniques to induce and identify specific senescence programs. Additional chapters describe cellular and mouse models in which is possible to study the complex cell and non-cell autonomous functions of senescent cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.