Next Generation Genome Sequencing: Towards Personalized Medicine

Written by leading experts from industry and academia, this first single comprehensive resource addresses recent developments in next generation DNA sequencing technology and their impact on genome research, drug discovery and health care. As such, it presents a detailed comparative analysis of commercially available platforms as well as insights into alternative, emerging sequencing techniques. In addition, the book not only covers the principles of DNA sequencing techniques but also social, ethical and commercial aspects, the concept of personalized medicine and a five-year perspective of DNA sequencing.Content: Chapter 1 Sanger DNA Sequencing (pages 1–11): Artem E. Men, Peter Wilson, Kirby Siemering and Susan ForrestChapter 2 Illumina Genome Analyzer II System (pages 13–28): Abizar Lakdawalla and Harper VansteenhouseChapter 3 Applied Biosystems SOLiD™ System: Ligation?Based Sequencing (pages 29–42): Vicki Pandey, Robert C. Nutter and Ellen PredigerChapter 4 The Next?Generation Genome Sequencing: 454/Roche GS FLX (pages 43–56): Lei Du and Michael EgholmChapter 5 Polony Sequencing: History, Technology, and Applications (pages 57–76): Jeremy S. EdwardsChapter 6 Next?Generation Sequence Data Analysis (pages 77–88): Leonard N. BloksbergChapter 7 DNASTAR's Next?Generation Software (pages 89–94): Tim Durfee and Thomas E. SchweiChapter 8 Real?Time DNA Sequencing (pages 95–101): Susan H. HardinChapter 9 Direct Sequencing by TEM of Z?Substituted DNA Molecules (pages 103–116): William K. Thomas and William GloverChapter 10 A Single DNA Molecule Barcoding Method with Applications in DNA Mapping and Molecular Haplotyping (pages 117–132): Ming Xiao and Pui?Yan KwokChapter 11 Optical Sequencing: Acquisition from Mapped Single?Molecule Templates (pages 133–151): Shiguo Zhou, Louise Pape and David C. SchwartzChapter 12 Microchip?Based Sanger Sequencing of DNA (pages 153–163): Ryan E. Forster, Christopher P. Fredlake and Annelise E. BarronChapter 13 Multiplex Sequencing of Paired End Ditags for Transcriptome and Genome Analysis (pages 165–182): Chia?Lin Wei and Yijun RuanChapter 14 Paleogenomics Using the 454 Sequencing Platform (pages 183–199): M. Thomas P. GilbertChapter 15 ChIP?Seq: Mapping of Protein–DNA Interactions (pages 201–215): Anthony Peter Fejes and Steven J. M. JonesChapter 16 MicroRNA Discovery and Expression Profiling using Next?Generation Sequencing (pages 217–228): Eugene Berezikov and Edwin CuppenChapter 17 DeepSAGE: Tag?Based Transcriptome Analysis Beyond Microarrays (pages 229–244): Kare L. Nielsen, Annabeth H. Petersen and Jeppe EmmersenChapter 18 The New Genomics and Personal Genome Information: Ethical Issues (pages 245–254): Jeantine E. Lunshof