Gel-Free Proteomics: Methods and Protocols
Gel-Free Proteomics: Methods and Protocols By Kenny Helsens, Lennart Martens, Joël Vandekerckhove, Kris Gevaert (auth.), Kris Gevaert, Joël Vandekerckhove (eds.)
2011 | 400 Pages | ISBN: 1617791474 | PDF | 10 MB
2011 | 400 Pages | ISBN: 1617791474 | PDF | 10 MB
Proteomics by means of mass spectrometry has rapidly changed the way that we analyze proteomes. Gel-Free Proteomics: Methods and Protocols addresses contemporary methods for gel-free proteome research with a special focus on differential analysis and protein modifications. Divided into twenty-five chapters, this detailed volume meticulously describes vital procedures needed to perform gel-free proteomics, ranging from sample preparation, isotope labeling for differential proteomics, enrichment technologies for modified proteins and peptides, and bioinformatics. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and easily accessible, Gel-Free Proteomics: Methods and Protocols serves as a timely resource for both professionals and novices pursuing research in this critical field.