Electrophoresis Complete Concepts

Detailed explanation

What you'll learn
Electrophoresis Introduction
Paper Electrophoresis
Paper Electrophoresis Applications
Agarose Gel Electrophoresis
Agarose Gel Electrophoresis Applications
Immuno Electrophoresis
Immuno Electrophoresis Applications
Iso Electrophoresis
Iso Electrophoresis Applications
Iso Electric focusing
Iso Electric focusing Applications
Electrophoresis Applications and conclusion
Requirements
Any Life science student can learn concepts
Description
Electrophoresis is a laboratory technique used to separate DNA, RNA, or protein molecules based on their size and electrical charge. An electric current is used to move molecules to be separated through a gel. Arne Tiselius in 1931, while new separation processes and chemical analysis techniques based on electrophoresis continue to be developed in the 21st century.Electrophoresis is a widely used analytical technique for the separation of biological molecules such as plasma proteins , lipoproteins and immunoglobulins.Electrophoresis IntroductionPaper ElectrophoresisPaper Electrophoresis ApplicationsAgarose Gel Electrophoresis Agarose Gel Electrophoresis ApplicationsImmuno Electrophoresis Immuno Electrophoresis ApplicationsIso Electrophoresis Iso Electrophoresis ApplicationsIso Electric focusingIso Electric focusing ApplicationsTypes: Zone Electrophoresis Paper electrophoresis Gel electrophoresis Immunoelectrophoresis Isoelectric focusingZone electrophoresis, A simple and modified method of moving boundary electrophoresis is the zone electrophoresis.An inert supporting material such as paper or gel are used.Paper  Electrophoresis It is a technique that use what man filter paper number 1.moisten by a buffer and then connected by two ends electrode Then the sample is applied on one end and left for separation of the component under electric field.After separation the filter paper is dried and stained to get coloured bands.These coloured bands are recognized for the natural sample by comparing with the standard. For a sample of sterol five band s of protein can be separated.and more concepts in explanations

Overview

Section 1: Introduction

Lecture 1 Introduction

Lecture 2 Paper Electrophoresis

Lecture 3 Paper Electrophoresis Applications

Lecture 4 Agarose Gel Electrophoresis

Lecture 5 Agarose Gel Electrophoresis Applications

Lecture 6 Immuno Electrophoresis

Lecture 7 Immuno Electrophoresis Applications

Lecture 8 Iso Electric Focusing

Lecture 9 Iso Electric Focusing Applications

Lecture 10 Electrophoresis Applications and conclusion

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