Uv-Visible Spectroscopy And Circular Dichroism Spectroscopy

Spectroscopy

What you'll learn

Basic Concept of UV-Visible spectroscopy.

Interaction of organic molecules with light and what do we measure in UV-Visible spectroscopy.

UV-Visible spectrum of ATP

UV-visible spectrum of NAD+/NADH

UV-Visible spectrum of cytochrome-c

UV-Visible spectrum of Beta-carotene

UV-Visible spectrum of Proteins

UV-Visible spectrum and melting point of DNA

UV-Visible spectrum and purity of DNA and proteins

Types of light and what is circular dichroism?

How are we measuring circular dichroism and introduction various parameters.

Circular dichroism of proteins

Circular dichroism of DNA

Additional learning on Ramachandran plot of proteins

Requirements

No previous skills are required

Enthusiasm to learn biology and techniques related to biology, biochemistry and organic chemistry

Description

The major issues with the courses on spectroscopy are inclusion of too much instrumentation, electronics, mathematical  calculations, and very less discussion on data analysis. This course on UV-Visible spectroscopy and circular dichroism spectroscopy for the students is for the students who want to learn the basic aspects and the data analysis by using so many examples. This course will be teaching about two major and important techniques related to biology and organic chemistry that is UV visible spectroscopy and circular dichroism spectroscopy. It is a perfect introductory course for the students which starts its journey with discussion on basic concept of visible spectroscopy and it tells you about how different organic molecules with double bonds, with conjugated systems, and with lone pairs are interacting with light. Then it starts discussing about different examples of UV visible spectroscopy data analysis with the help of graphs. In the third section, it talks about circular dichroism by starting with types of light and what is actually circular dichroism. Then it tells you how we are measuring CD, circular dichroism, what is ellipticity, basic formulas, basic discussion of fundamentals, and basic mathematics of CD. Then it goes on and talks about circular dichroism of proteins by taking 3 to 4 examples. Then finally, the third section ends with circular dichroism of DNA. This course also contains a additional learning section where you learn about a very interesting but complicated topic, The Ramachandran plot of protein secondary structures. So, guys, let us enjoy the whole discussion on UV visible spectroscopy and circular dichroism spectroscopy. All the best.

Overview

Section 1: Introduction to the course

Lecture 1 Introduction

Section 2: UV-Visible Spectroscopy

Lecture 2 Basic concept of UV-Visible Spectroscopy

Lecture 3 Interactions of organic molecules with UV-Visible light

Lecture 4 What do we look in UV-Visible spectrum

Lecture 5 UV-Visible spectrum of ATP

Lecture 6 UV-Visible spectrum of NAD+/NADH

Lecture 7 UV-Visible spectrum of Cytochrome c

Lecture 8 UV-Visible spectrum of Beta-Carotene

Lecture 9 UV-Visible spectrum of proteins

Lecture 10 UV-Visible spectroscopy and melting point of DNA

Lecture 11 UV-Visible spectroscopy and purity of DNA and proteins

Lecture 12 Reference readings

Section 3: Circular Dichroism Spectroscopy

Lecture 13 Basic Beer-Lambert law

Lecture 14 Types of lights - Unpolarized, plane polarized and circularly polarized light

Lecture 15 What is circular dichroism

Lecture 16 How do we measure circular dichroism

Lecture 17 Ellipticity as the measurement of circular dichroism

Lecture 18 Circular dichroism of proteins - 1

Lecture 19 Circular dichroism of proteins -2

Lecture 20 Circular dichroism of proteins -3

Lecture 21 Circular dichroism of DNA

Lecture 22 References to read circular dichroism spectroscopy

Section 4: Additional learning - Ramachandran Plot for proteins

Lecture 23 Ramachandran plot

Bachelor's students of biology, organic chemistry, biochemistry and life sciences.,Master's students of biology, organic chemistry, biochemistry and life sciences.,Researchers in the field of biology, organic chemistry, biochemistry and life sciences.,Medical students and medical professionals,Biology, organic chemistry, biochemistry and life science enthusiasts