Master Primer Designing & Polymerase Chain Reaction (Pcr)

Learn Primer Designing and In-silico Polymerase Chain Reaction(PCR) amplification using Bioinformatics Tools.

What you'll learn
You will learn Brief Introduction to PCR Including (Principle, Procedure, Stages, and Optimization)
You will learn Applications of PCR
You will learn Advantages & Limitations of PCR
You will learn Variations and History of PCR
You will learn Primer Designing | Primer Specification or Primer Requirements
You will learn Primer Designing (Manually) by ClustalW & Oligocalculator
You will learn about Primer3 tool | Automated method for Primer Designing
You will learning about Primer validation by BLAST
You will learn In_silico PCR | How to confirm PCR amplifiacion
Requirements
Basic Understanding of Biology and Bioinformatics
Willingness to learn
Good Internet and notebook to note down the lectures
There is no coding in this course. Most of tools which we will use that's available online
Description
In this course you will learn:The basic concepts of the standard polymerase chain reaction (PCR) technique.The criteria required to design a DNA primer for PCR.The online programs we usually use to design a DNA primer.Polymerase chain reaction (PCR)You will learn in this section:Steps involved in the PCR (PCR cycling): denaturation, annealing, extensionComponents of the PCR reaction: DNA template, DNA polymerase, dNTPs, forward primer and reverse primer.PCR amplification program.Exponential amplification.The size difference between the PCR amplification products of the first, second, and third cycle.Criteria for PCR primer designYou will learn in this section a detailed explanation of the PCR primer design criteria and how they affect the primer sensitivity and stability including; primer length, primer melting temperature, primer annealing temperature, GC% of the primer, GC-clamp, cross homology and primer secondary structure.Tools and methodsIn this section you will learn how to:Retrieve a Gene Sequence form NCBI, and Determine the Exact Location for Each Exon on the Chromosome Using Graphics.Compare Different mRNA Transcripts and Select One to Evaluate a Gene Expression in Novel Cells.Understand Primer3 Setting.Calculate Primer Self-Complementarity Score.Check for Primer Cross Homology Using BLAT.In_silico PCR, How to confirm PCR amplification

Overview

Section 1: Introduction to Polymerase chain reaction

Lecture 1 What is Polymerase chain reaction (PCR)?

Lecture 2 Principles and Procedure of Polymerase chain reaction (PCR)

Lecture 3 Stages and Optimization of Polymerase chain reaction (PCR)

Lecture 4 Applications of Polymerase chain reaction (PCR) Part 01

Lecture 5 Applications of Polymerase chain reaction (PCR) Part 02

Lecture 6 Advantages & Limitations of Polymerase chain reaction (PCR)

Lecture 7 History of Polymerase chain reaction (PCR)

Lecture 8 Variations of Polymerase chain reaction (PCR)

Section 2: Primer Designing

Lecture 9 What is a Primer?(Primer Introduction)

Lecture 10 PCR primer design

Lecture 11 Primer Specification or Primer Requirements (Criteria)

Lecture 12 Primer Designing (Manually) by ClustalW & Oligocalculator

Lecture 13 Primer3 tool | Automated method for Primer Designing

Lecture 14 Primer validation by BLAST

Section 3: In_silico PCR

Lecture 15 In_silico PCR | How to confirm PCR amplifiacion

Bioinformaticians & Biotechnologists are Encouraged to take this course.,Entry Level Primer Designer,Researchers are also encouraged to take this Course,Genetic laboratory professionals who work on PCR workbench to gain additional knowledge,Anyone who want to learn something New