Molecular Analysis and Genome Discovery

This advanced level textbook provides a comprehensive overview of recent developments in the area of molecular based diagnostics (including nucleic acids, biosensors and immunoessays) of disease markers. It also covers the impact of techniques such as in vitro nucleic acid amplifications (e.g. PCR) and other amplification methods, as well as gene and biochip production and automated techniques such as fluorescent sequencing. The book discusses key concepts where new and merging areas, including pharmacogenomics, proteomics and functional genomics, are being researched and developed. In addition, examples are given where this new area of bioscience has or may be successfully applied.Content: Chapter 1 Pharmacogenetics and Pharmacogenomics: An Overview (pages 1–15): W. KalowChapter 2 Quantitative TaqMan Real?Time PCR: Diagnostic and Scientific Applications (pages 17–28): Jorg Dotsch, Ellen Schoof and Wolfgang RascherChapter 3 Hybridization Probes for Real?Time PCR (pages 29–42): Elaine LyonChapter 4 An Overview of Genotyping and Single Nucleotide Polymorphisms (SNP) (pages 43–69): Ivo Glynne GutChapter 5 High?Throughput Mutation Screening (pages 71–100): Paal Skytt Andersen and Lars Allan LarsenChapter 6 Determination of Nucleic Acid Sequences by Pyrosequencing (pages 101–111): Elahe Elahi and Mostafa RonaghiChapter 7 An Introduction to DNA Chips (pages 113–126): Magdalena Gabig?Ciminska and Andrzej CiminskiChapter 8 Overview of Microarrays in Genomic Analysis (pages 127–165): Janette K. BurgessChapter 9 Overview of Differential Gene Expression by High?Throughput Analysis (pages 167–190): Kin?Ying ToChapter 10 Aptamers: Powerful Molecular Tools for Therapeutics and Diagnostics (pages 191–215): Eva Baldrich Rubio, Monica Campas i Homs and Ciara K. O'SullivanChapter 11 Chip Based Proteomics Technology (pages 217–249): Mikhail Soloviev, Richard Barry and Jon TerrettChapter 12 Infectomics Overview: Holistic and Integrative Studies of Infectious Diseases (pages 251–270): Sheng?He Huang and Ambrose JongChapter 13 The Drug Discovery Process (pages 271–294): Roberto SolariChapter 14 Structure?Based Drug Discovery (pages 295–321): Chen?Chen Kan, Kevin Hambly and Derek A. DebeChapter 15 Protein Interaction–Targeted Drug Discovery (pages 323–345): Gary Hudes, Sanjay Menon and Erica A. GolemisChapter 16 Overview of Quantitative Structure–Activity Relationships (QSAR) (pages 347–367): David A. Winkler