Structural Proteomics And Its Impact On The Life Sciences

The role played by structural proteomics in the first decade of the 21st century is equivalent to that played by the Human Genome Project in the last decade of the 20th century. The development of high-throughput technologies that permit the solution of hundreds of 3D structures of individual proteins, protein protein complexes and protein drug complexes, just by one laboratory in a single year, will provide a knowledge base which will change the face of structural biology. This will have an immediate influence on medicinal chemistry and molecular pharmacology, as well as an increasing impact on such disciplines as neurobiology, developmental biology, immunology and molecular medicine. This book presents a state-of-the-art overview of the structural proteomics field, ranging from policy issues related to funding and goals, through the high-throughput procedures for protein production, to the solution of the structures of proteins and higher-order entities, via a multidisciplinary approach involving molecular biology, X-ray crystallography, NMR and electron microscopy, as well as bioinformatics analysis. This is the first book to provide such a comprehensive coverage of a rapidly evolving field. Contents: The Importance of Target Selection Strategies in Structural Biology (E E Abola & R C Stevens); The Impact of Structural Proteomics on Macromolecular Structure Databases (J D Watson & J M Thornton); The Impact of 3D Structures on a Protein Knowledgebase: From Proteins to Systems (U Hinz & A Bairoch); Bioinformatics of Protein Function (A M Lesk et al.); Comparative Modeling in Structural Genomics (J Moult); The Contribution of Structural Proteomics to Understanding the Function of Hypothetical Proteins (M D Suits et al.); Intrinsically Disordered Proteins (P Tompa); Metalloproteins: Structure, Conservation and Prediction of Metal Binding Sites (M Edelman et al.); The Impact of Protein Expression Methodologies on Structural Proteomics (A Chesneau et al.); Protein Complexes Assembly by Multi-Expression in Bacterial and Eukaryotic Hosts (C Romier); The Impact of Structural Proteomics on the Prediction of Protein-Protein Interactions (C Kiel & L Serrano); Cryo-Electron Microscopy in the Era of Structural Proteomics (A C Steven & D M Belnap); On NMR-based Structural Proteomics (T Szyperski); Structural Proteomics in Relation to Signaling Pathways (F Bedez et al.); The Impact of Structural Proteomics and Drug Design (Y-P Pang); Structural Proteomics of Emerging Viruses: The Example of SARS-CoV and Other Coronaviruses (R Hilgenfeld et al.); High-throughput Technologies for Structural Biology: The Protein Structure Initiative Perspective (A Joachimiak); European Structural Proteomics A Perspective (S Daenke et al.); Structural Genomics and Structural Proteomics: A Global Perspective (L Banci et al.); Policies in Structural Genomics/Structural Proteomics (J Norvell & J Berg).