A panel of highly regarded molecular biologists and clinical researchers describe in detail their most novel, useful, and interesting RT-PCR applications. Here the newcomer will find readily reproducible protocols for highly sensitive detection and quantification of gene expression, the in situ localization of gene expression in tissue, and the cloning of genes, as well as for analyzing T-cell clones and the differential expression of genes. For the expert seeking to extend the usefulness of RT-PCR, there are user-friendly applications that complement the latest technological advances, including laser-capture microdissection (LCM), real-time and quantitative PCR, microarray technology, cDNA cloning, and antibody engineering.

Bert Jansch, legendary songwriter and guitarist, is widely acknowledged as one of the most influential musicians of all time. Since the mid 1960s generation after generation have been enraptured and amazed by his extraordinary ground-breaking guitar playing and classic emotive songs. Jimmy Page, Neil Young, Nick Drake, Johnny Marr, Bernard Butler and Beth Orton have all been devotees, and today another new generation of musicians and fans, led by Devendra Banhart, are discovering Jansch for themselves.

Bert began performing his unique synthesis of folk, blues and jazz on the folk club scene of the early 1960s, having hitch-hiked to London from his hometown of Edinburgh. His first album, Bert Jansch (played on a borrowed guitar and recorded on a reel-to-reel tape deck in someone's apartment) was legendarily sold to the Transatlantic label for ú100. On its release in April 1965, Bert Jansch caused a sensation for its innovative guitar technique and powerful songs, and it has been phenomenally influential to this day, cited by legions of guitar players (famous and otherwise) as a major inspiration.

Bert's new album The Black Swan was released on Sanctuary on September 18 (except USA and Canada) and has been receiving fantastic reviews across Europe. Producer Noah Georgeson (Joanna Newsom – Milk-Eyed Mender, Devendra Banhart – Cripple Crow) worked with Bert on the record, as well as a number of guests including Devendra Banhart, Beth Orton, Otto Hauser (Espers, Vetiver), Helena Espvall (Espers), Kevin Barker (Currituck Co.), Paul Wassif and Adam Jansch.